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KMID : 0381120140360040443
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Genes and Genomics
2014 Volume.36 No. 4 p.443 ~ p.453
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Up-regulation of the arginine vasotocin precursor gene from Paralichthys olivaceus: isolation and expression upon acute pathogen invasion
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Lee Seong-Do
Lim Bong-Soo
Lee Je-Hee
Kim Yu-Cheol
Kim Hyo-Won
Priyathilaka Thanthrige Thiunuwan
Wickramaarachchi W. D. Niroshana
Kim Se-Jae
Kim Shin-Kwon
Jeong Hyung-Bok
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Abstract
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Arginine vasotocin (AVT) is a neurohypophysial hormone of non-mammal vertebrates functions in various physiological processes and homologous to mammal vasopressin. In this study, a full-length cDNA coding for a putative AVT precursor was isolated from flounder, Paralichthys olivaceus by rapid amplification of cDNA ends. It was 641 bp long, coding 153 amino acids, and consisted in 69 bp long 5′-untranslated region (UTR), 462 bp open reading frame and 110 bp 3′-UTR. The putative amino acid sequence of flounder AVT was determined, and compared with other AVTs from different species. Also, 1,447 bp of flounder AVT gene, corresponding to the isolated cDNA region with 2 introns within it, was isolated. Reverse transcription polymerase chain reaction result showed that flounder AVT was strongly expressed in the brain but not in the any other tissues examined, confirming it is brain-specific gene. Expression profile of the AVT, together with arylalkylamine N-acetyltransferase, heat shock protein 70, interferon-stimulated gene 15 and natural resistance-associated macrophage protein genes were surveyed from Streptococcus iniae infected flounders by real-time quantitative PCR. It revealed that flounder AVT is up-regulated under acute stress compared to the non-challenged control.
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KEYWORD
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Paralichthys olivaceus, Arginine vasotocin (AVT), Cloning, Expression, Gene structure, Pathogen challenge, Real-time quantitative PCR
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